DNA/RNA precipitation

To a solution containing DNA/RNA add the following components:

1/10 vol. of Sodium Acetate 3 M (pH 5.0)

20-50 µg glycogen (optional)

2 vol. of absolute EtOH or 1 vol. of isopropanol

  • Mix well and let stand overnight at –20oC or for 20 min. at –70oC

  • Precipitate nucleic acid by centrifuging at max. speed for 15 min

  • Pour out supernatant and add 500 µl EtOH 75%. Centrifuge for 2 min. at max. speed

  • Pour out supernatant and air dry the pellet